This website is free and open to all users, and there is no login requirement.
PrimerScore is a robust high-throughput primer design tool that designs multiple types of primers in one click, including face-to-face, back-to-back, and nested primers, as well as evenly full-covered primers on target regions. PrimerScore precisely evaluates candidate oligos′features, containing specificity, SNPs, dimers, and other basic features. Then it scores the oligos and pairs according to the features using growth curve piecewise function and weighted sum model. Finally, it provides the highest-scoring primer pairs or probes and examines for cross-products and cross-dimers among the outputted primers.
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